Effective Chromosomal Preparation Protocol for the Dioecious Plant <i>Silene latifolia</i>

Cytology requires chromosome specimens, thus, effective preparation methods are needed. Chromosome specimens frequently prepared from plant root tips. Furthermore, cell cycle synchronization using chemical reagents is applied to obtain a large number of metaphase specimens. In this study, we focused on the timing tip sampling, which optimal for dioecious Silene latifolia. The was determined time seeds were subjected germination treatment. Observation chromosomes microscopy revealed that mitotic cells peaked 54 h after This trend also observed when DNA synthesis inhibitor aphidicolin administered 24 9 before sampling points. We used ice-cold treatment 8, 16, and 32 as condensation method. 16 produced suitable showing satellite ends chromosomes, whereas well-condensed counting numbers. Our findings suggest essential effectively producing